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Human/Mouse/Rat Phospho-SMAD2 PharmaGenie ELISA Kit
SKU: 00013603
- Brand:
- Assay Genie
Discount -1.25%
986.51 €
999.00 €
no VAT
Price subject to possible modifications
Pack/Lote: N/D
Stock: 0 available
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Estimated delivery time: 17 days.
Manufacturer code
SBRS1970
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Unit
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500g
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Not available
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Extended description
Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) PharmaGenie ELISA Kit
Assay Genie Phospho-SMAD2 (Ser245/250/255) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated SMAD2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-SMAD2. An anti-pan SMAD2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SMAD2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-SMAD2 (Ser245/250/255) antibody is used to detect phosphorylated SMAD2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SMAD2 (Ser245/250/255) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
| Product SKU: | SBRS1970 |
| Size: | 96T |
| Application: | The antibody pair provided in this kit recognizes human, mouse, rat SMAD2 phosphorylated at site Serine-245/250/255. |
| Uniprot: | Q15796 |
| Gene ID: | 4087 |
| Gene Names: | MADH2 / MADR2 / SMAD2 |
| Pathway: | Angiogenesis / Stem Cell / TGF-beta Signaling |
| Synonyms: | Mothers against decapentaplegic homolog 2 (MAD homolog 2) (Mothers against DPP homolog 2) (JV18-1) (Mad-related protein 2) (hMAD-2) (SMAD family member 2) (SMAD 2) (Smad2) (hSMAD2) |
| Target Species: | Human, Mouse, Rat |
| Compatible Sample Types: | Cell Lysates, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Storage/Stability: | Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at -20°C. Item D, store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
Other materials and equipment required:
The Assay Genie Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) PharmaGenie ELISA Kit (SBRS1970) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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