ALPHA.SF ULTRA CDK1 P-T14, 500

Cyclin-dependent kinase 1 (CDK1), encoded by the CDC2 gene, is a serine/threonine kinase ubiquitously expressed in eukaryotes. CDK1 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis. Dysregulated activation of CDK1 leads to uncontrolled cell division and is a hallmark of cancer. Inhibition of CDK1 is an active research area, especially with new approaches involving PROTACs.The AlphaLISA SureFire Human Phospho-CDK1 (Thr14) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-CDK1 (Thr14) in cellular lysates, using Alpha Technology.FormatsThe HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.AlphaLISA SureFire Ultra kits are compatible withCell and tissue lysatesAntibody modulatorsBiotherapeutic antibodiesAlpha SureFire kits can be used forCellular kinase assaysReceptor activation studiesScreening