ALPHA.SF ULTRA ATF-4 TOTAL, 50K

Activating transcription factor 4 (ATF-4), also known as tax-responsive enhancer element B67, is a multifunctional transcription regulatory protein in the basic leucine zipper superfamily. ATF-4 is a member of a family of DNA-binding proteins that includes AP-1 family, CREBs, and CREB-like proteins, all of which share a leucine zipper region that is involved in protein-protein interactions. Under stress conditions, PERK pathway rapidly stimulates the expression of NRF2 through activating transcription factor 4 (ATF4) that contributes to the NRF2 protective response.The AlphaLISA SureFire Human and Mouse Total ATF-4 Detection Kit is a sandwich immunoassay for the quantitative detection of total ATF-4 in cellular lysates, using Alpha Technology.FormatsThe HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume. AlphaLISA SureFire Ultra kits are compatible withCell and tissue lysatesAntibody modulatorsBiotherapeutic antibodiesAlpha SureFire kits can be used forCellular kinase assaysReceptor activation studiesScreening